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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 17-20.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

HBV RNA复制子疫苗、DNA疫苗在BHK-21细胞中表达情况的比较

李莉,任林柱,张英,杨东,闫森,李小平,高飞,肖雪斌,欧阳红生,逄大欣   

  • 收稿日期:2009-12-10 修回日期:2010-03-02 出版日期:2010-05-20 发布日期:2010-05-20

Detecting the expression efficiency of the RNA replicon vaccines and DNA vaccine of HBV in BHK-21 cells

Li Li, Ren Linzhu, Zhang Ying, Yang Dong, Yan Sen, Li Xiaoping,   

  • Received:2009-12-10 Revised:2010-03-02 Online:2010-05-20 Published:2010-05-20

PDF (PC)

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摘要：

摘要：通过将所构建的HBV RNA疫苗和DNA疫苗分别转染BHK-21细胞，并比较其在细胞中表达情况及细胞凋亡现象。结果表明HBV RNA疫苗比DNA疫苗表达效果更好，安全性更高，更具有应用前景。

关键词: 牡丹, 牡丹, AM菌根, 高温胁迫, 生理生化

Abstract:

Abstract: Construction of RNA replicon vaccine and DNA vaccine of HBV, then the 2 vectors were tranfected into BHK-21 cells and their expression efficiency was detected by ELISA with the kits used to detect HBsAg and preS2. Meantime, we found that the vectors based on pSFV can make BHK-21 cells dead (apoptosis). It is confirmed that the immune effect of RNA replicon vaccine is better than that of DNA vaccine. RNA vaccine is safer and more prospects.