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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 14-16.

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

犬精液冷冻方法的优化试验

张志平,张君涛,牛森,丁佩佩,林峰   

  • 收稿日期:2009-11-10 修回日期:2010-01-04 出版日期:2010-05-20 发布日期:2010-05-20

Optimized Test of Canine Semen Freezing Methods

Zhang Zhiping, Zhang Juntao, Niu Sen, Ding Peipei, Lin Feng   

  • Received:2009-11-10 Revised:2010-01-04 Online:2010-05-20 Published:2010-05-20

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摘要：

摘要：本试验为提高犬的精液冷冻效果，冷冻保存液配方中添加不同浓度糖（葡萄糖，果糖），平衡时间以及熏蒸时间做系统对比。经系列分组试验发现，含0.5 %果糖组中精子活力显著高于其它组别（P<0.05）；平衡时间120 min（P<0.05）；熏蒸时间20 min冷冻效果较好。结果表明犬精子冷冻液添加低浓度果糖优于葡萄糖，而且平衡时间为120 min，熏蒸时间为20 min可得到稳定的冷冻效果。关键词：犬；:精子；葡萄糖；果糖；:冷冻保存

关键词: 农技协, 农技协, 发展, 见解

Abstract:

Abstract：The study was conducted to cryopreserve canine semen for optimizing cryopreservation medium. The different concentration of sugar (Glucose Fructose), different balance times, different fumigation times were selected systematically in the cryopreservation medium. The results showed addition of 0.5% fructose was better than glucose at any concentration(P<0.05). 120 min balance time and 20 min liquid nitrogen fumigation time were excellent for motility of frozen sperm (P<0.05). In conclusion, the optimized procedures(0.5% fructose, 120 min balance time and 20 min ) were feasible and steady for freezing canine sperm. Key words：Canine, Sperm, Glucose, Fructose, Cryopreservation