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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 330-335.

所属专题： 资源与环境； 食用菌； 食用菌； 水产渔业

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

鲍鱼菇（Pleurotus abalonus）种质资源SCAR标记的建立及其初步应用

熊芳,郑闽江,刘新锐,谢宝贵   

  • 收稿日期:2010-01-13 修回日期:2010-02-11 出版日期:2010-06-05 发布日期:2010-06-05
  • 基金资助:

    福建省科技重大专项资助项目

Establishment and preliminary application of scar marker in germplasm resource of Pleurotus abalones

Xiong Fang, Zheng Minjiang, Liu Xinrui, Xie Baogui   

  • Received:2010-01-13 Revised:2010-02-11 Online:2010-06-05 Published:2010-06-05

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摘要：

为有效解决鲍鱼菇市场菌种名称异常混乱的现象，本文对全国16株常用鲍鱼菇栽培菌株进行SRAP-PCR、RAPD-PCR扩增，从电泳图谱上选取特异片段，成功转化成SCAR标记，通过构建的4对SCAR 特异性引物，将这16株鲍鱼菇菌株分为5 类，有效地解决了鲍鱼菇市场上常见的“同种异名、同名异种”现象。为鲍鱼菇核心种质的构建和资源的科学保存提供了依据，并为规范生产和产品质量标准的制定奠定了基础。

关键词: 河南, 河南, 优质小麦, 结构, 分析

Abstract:

In order to solve the chaotic phenomena of naming Pleurotus abalonus variously in the market, this article mainly analyzes 16 Chinese regular Pleurotus abalonus cultivating bacterial strains by SRAP-PCR and RAPD-PCR augmentation, and then choose the special segments from electrophoretogram, transform the segment into SCAR mark, and further classify these 16 bacterial strains into 5 categories through the 4 pairs of SCAR particular primer, so as to settle the chaos of giving various names to the same kind or going to the contrary in the abalone mushroom market. This article not only provides the evidence for the foundation of the core classification of Pleurotus abalonus and the scientific record of the relevant resources, but also lays a foundation for the product standard rate establishment and for the standardized manufacture.