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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 1-4.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    下一篇

Smad2在附植后早期绵羊子宫内膜和滋养层的分布

贺俊平,文雪雪,田志英,赵鹂,张学敬   

  • 收稿日期:2010-01-29 修回日期:2010-03-15 出版日期:2010-06-20 发布日期:2010-06-20
  • 基金资助:

    山西省青年科技研究基金

The Distribution of Smad2 in Early Post-implantation Ovine Endometrial and Trophoblast

He Junping, Wen Xuexue, Tian Zhiying, Zhao Li, Zhang Xuejing   

  • Received:2010-01-29 Revised:2010-03-15 Online:2010-06-20 Published:2010-06-20

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摘要：

Smad2是TGFβ超家族蛋白的胞质内信号转导分子，以往的研究证实Smad2的mRNA表达和定位于大鼠、小鼠子宫。本项研究的目的是用免疫组织化学方法检测Smad2蛋白在附植早期（妊娠32天）绵羊子宫内膜的分布。选山西省太谷县本地绵羊，于秋季交配后32天（32 day post coitus, dpc），解剖取材子宫和胎衣组织，制作石蜡切片，用免疫组织化学方法检测了Smad2蛋白在子宫内膜的分布。结果显示Smad2蛋白定位于子宫腔上皮、腺上皮和上皮下方的基质中；胚胎附植处Smad2高表达，胎衣滋养层组织也呈现Smad2强阳性表达。结论：Smad2表达于附植早期绵羊子宫内膜和滋养层，可能参与子宫内膜和附植后胚胎发育的调节。

关键词: 蝴蝶兰, 蝴蝶兰, 高温胁迫, 渗透调节物质, 细胞保护酶活性, 超氧化物自由基, 膜脂过氧化, 质膜相对透性

Abstract:

Smad2 was the intracellular signal transducer of transforming growth factorβ (TGFβ) superfamily proteins. Previous studies confirmed that Smad2 mRNA expressed in the rat and mouse uterus during implantation period. The present study examined Smad2 protein expression and localization in sheep endometrial and trophoblast during the early post-implantation period by the method of immunohistochemistry. Results showed that Smad2 protein was predominantly present in the luminal epithelial, glandular epithelium, and trophoblast. These results suggest that Smad2 expressed in the uterus and trophoblast, might be involved in the regulation of the development of endometrial and trophoblast.