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中国农学通报 ›› 2018, Vol. 34 ›› Issue (24): 6-12.doi: 10.11924/j.issn.1000-6850.casb17060055

所属专题: 玉米

• 农学 农业基础科学 • 上一篇    下一篇

低温胁迫对玉米幼苗抗氧化系统及渗透调节物质的影响

黄伟超,范宇博,王泳超   

  1. 浙江出入境检验检疫局,浙江省杭州市,浙江出入境检验检疫局,浙江省杭州市,河南农业大学农学院
  • 收稿日期:2017-06-08 修回日期:2017-07-11 接受日期:2017-07-21 出版日期:2018-08-28 发布日期:2018-08-28
  • 通讯作者: 王泳超
  • 基金资助:
    浙江省出入境检验检疫局内部项目“实蝇、舞毒蛾的快速侦测及预警体系建立”(2014-ZKZ-014)。

Low Temperature Stress and Maize Seedlings: Effect on Antioxidant Enzyme System and Osmotic Regulation

  • Received:2017-06-08 Revised:2017-07-11 Accepted:2017-07-21 Online:2018-08-28 Published:2018-08-28

摘要: 为了研究低温胁迫下不同基因型玉米幼苗抗氧化系统及渗透调节物质的差异响应,列出主要的实验方法。选用‘先玉335’和‘丰禾1号’为供试品种,设置了常温对照(CK)和低温处理(L),采用盆栽方式种植,随机设计。结果如下:低温胁迫会影响玉米形态的建成,降低株高、叶面积、总干重、根长、根表面积和根体积,且‘先玉335’的指标下降值小于‘丰禾1号’。低温胁迫下叶片内的丙二醛(MDA)和超氧阴离子(O2.-)含量会明显升高,‘先玉335’的积累量小于‘丰禾1号’。随着有害物质的积累,叶片内的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性随之升高,以便清除有害物质,保护细胞膜。‘先玉335’的酶活性在低温胁迫时上升的快于‘丰禾1号’。可溶性蛋白、可溶性糖和脯氨酸含量在低温胁迫下会升高,这有利于维持细胞膨压,减少低温损伤。玉米会调节自身生理代谢,从而减少低温带来的损伤。

关键词: 大豆, 大豆, 农杆菌介导, 遗传转化

Abstract: In order to research effect of low temperature stress on antioxidant enzyme system and osmotic regulation of different genotypes maize seedlings. Resistant variety (xianyu 335) and sensitive variety (fenghe 1) were selected in the research and room temperature treatment (CK) and low temperature treatment (L) were set. Potted plants were used in the research, and random design. The aim of research is to provide a theoretical basis for study of cold resistance physiological. The results are as follow: maize morphogenesis was affected by low temperature, and plant height, leaf area, dry matter, root length, root surface area and root volume were decrease. Compared to ‘fenghe 1’, ‘xianyu 335’ showed less descending value in the above indicators. MDA and和O2.- content were increased significantly, and ‘xianyu 335’ showed less accumulation of malondialdehyde (MDA) and superoxide anion (O2.-) content than ‘fenghe 1’. With the accumulation of harmful substance, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity in leaf were increased which could remove harmful substances, protect the cell membrane. The enzyme activity of ‘xianyu 335’ rose faster than ‘fenghe 1’ in low temperature stress. Low temperature stress could increase soluble protein, soluble sugar and proline, which was benefited for maintaining the pressure in cell, reducing damage. Physiological metabolism of maize could be regulated for reducing low temperature damage.