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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (16): 262-266.

所属专题： 生物技术； 园艺

• 植物保护 农药 • 上一篇    下一篇

侵染南瓜的西瓜花叶病毒和黄瓜花叶病毒CP基因的克隆和序列分析

刘金亮 王凤婷 魏毅 张世宏 潘洪玉   

  • 收稿日期:2010-03-09 修回日期:2010-04-15 出版日期:2010-08-20 发布日期:2010-08-20
  • 基金资助:
    “十一五”国家科技支撑计划;吉林大学博士科研启动基金;吉林大学基本科研业务费项目

Cloning and sequence analyses of the coat protein genes of Watermelon mosaic virus and Cucumber mosaic virus from one mix-infected squash plant

  • Received:2010-03-09 Revised:2010-04-15 Online:2010-08-20 Published:2010-08-20

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摘要： 采用RT-PCR的方法，用马铃薯Y病毒属病毒3′-末端序列的简并引物和黄瓜花叶病毒（Cucumber mosaic virus, CMV）外壳蛋白（CP）基因的特异引物，对采自山东聊城的一表现花叶、黄化、蕨叶及果实畸形的南瓜样品进行了检测，同时扩增到了西瓜花叶病毒（Watermelon mosaic virus, WMV）和CMV 2种病毒的基因组片段，说明该样品受到WMV和CMV 2种病毒的复合侵染。这2个病毒分离物分别被命名为WMV-liaocheng和CMV-liaocheng。序列测定及分析结果表明，它们与其它相应病毒分离物CP基因核苷酸序列的同源性分别为91.2-98.0%和77.0-97.9%，推导的氨基酸序列同源性分别为96.4-98.5%和81.2-99.1%。根据完整CP 基因核苷酸序列构建的系统进化树显示：18个WMV分离物可分为3组，其中WMV-liaocheng 与HLJ、CHN及Habenaria等分离物表现出较近的亲缘关系，形成Ⅲ组；30个CMV分为两个亚组，其中CMV-liaocheng属于亚组I，CMV-liaocheng可能发生过重组。

关键词: 氮, 氮, 磷, 钾

Abstract: Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV) were detected from one mix-infected pumpkin（Cucurbita moschata）plants in Liaocheng, Shandong Province. The nucleotide sequences of coat protein (CP) gene and the 3′-untranslated regions (3'-UTR) of WMV (WMV-liaocheng) and CP gene of CMV (CMV-liaocheng) were determined from RT-PCR derived cDNA clones. Compared with sequences of corresponding viruses that are available in the GenBank, the cp genes of WMV-liaocheng and CMV-liaocheng shared similarity of 91.2-98.0%, 77.0-97.9% at nucleotide level, and identity of 96.4-98.5%, 81.2-99.1% at amino acid level, respectively. The phylogenetic trees constructed with the complete nucleotide sequence of the CP genes showed that 18 WMV isolates were clustered into 3 groups and WMV-liaocheng belonged to group Ⅲ; 30 CMV isolates were divided into 2 subgroups, and CMV-liaocheng, Fny, Ixora, O, Y and NT9 were in subgroup I (CMV-I). There were probably recombination event during the evolution of CMV-liaocheng.