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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (14): 273-276.

所属专题： 小麦

• 植物保护 农药 • 上一篇    下一篇

小麦白粉菌拮抗真菌的筛选、鉴定及生防效果研究

伊艳杰 张长付 时 玉 赵 江   

  • 收稿日期:2010-03-15 修回日期:2010-04-15 出版日期:2010-07-20 发布日期:2010-07-20
  • 基金资助:
    小麦内生真菌拮抗病原菌的研究

Idenfication of Endophytic Fungi Antagonistic to Blumeria graminis and study on Biocontrol Efficacy

  • Received:2010-03-15 Revised:2010-04-15 Online:2010-07-20 Published:2010-07-20

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摘要： 从小麦茎叶组织中分离内生真菌，通过小麦白粉菌的孢子萌发抑制实验，共得到4株对小麦白粉菌孢子萌发抑制效果较好的菌株，抑制率分别达到86.08%、83.12%、91.14%和96.23%。利用小麦离体叶段白粉病害防治试验测定不同拮抗菌株的发酵液防治效果，根据白粉病病情鉴定的6级分类标准鉴定小麦病级，并计算病情指数和防治效果。其中拮抗菌株BF11和BF19处理后，小麦白粉病病情有所减轻，防治效果分别为：82.14%和88.49%。形态学初步鉴定结果表明，BF11为镰孢霉属（Fusarium sp.）真菌，BF19为曲霉属（Aspergillus sp.）真菌。

关键词: 高温, 高温, 高羊茅, 生理指标

Abstract: Endophytic fungi strains were isolated from stem and leaf of wheat. According to the ability to inhibit asexual spore germination on bearing glass, 4 isolates with antagonism were singled out and their control efficiency achieve to 86.08%, 83.12%, 91.14% and 96.23%, respectively. Fermentation filtrate of 4 strains were used to curb the disease development on detached wheat leaves. State of illness was identified according to the six standard and the Ind (%) were calculated. The results showed that BF11 and BF19 had better preventing efficacy for Blumeria graminis. The state of illness about wheat powdery mildew alleviated and biocontrol efficacy of BF11 and BF19 were 82.14% and 88.49%. The characteristics of morphology suggested that the strain of BF11 was identified as Fusarium and BF19 was identified as Aspergillus.