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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (16): 195-198.

所属专题： 生物技术

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

胶园球囊霉素相关土壤蛋白质及有机质特征

邓万刚 吴蔚东 林钊沐 祝 飞   

  • 收稿日期:2010-03-16 修回日期:2010-04-19 出版日期:2010-08-20 发布日期:2010-08-20
  • 基金资助:
    国家自然科学基金项目;海南省自然科学基金项目;海南省教育厅高等学校科研项目;中国热带农业科学院橡胶研究所2009年基本科研业务费

Study on character of glomalin-related soil protein and soil organic matter in rubber fields

  • Received:2010-03-16 Revised:2010-04-19 Online:2010-08-20 Published:2010-08-20

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摘要： 球囊霉素（glomalin）是土壤中一种含量丰富、极不寻常的蛋白质。本研究用20 mmol.L-1的热柠檬酸钠（121 ℃）提取海南白沙不同利用历史橡胶林剖面土壤中的球囊霉素相关土壤蛋白质，用Bradford染色法测定球囊霉素相关土壤蛋白质。研究结果表明，海南白沙橡胶林土壤中球囊霉素相关土壤蛋白质含量较低。相差8年的橡胶种植历史对土壤中球囊霉素相关土壤蛋白质含量的影响不显著。与第一代橡胶林(BFR、QFR) 各层次之间的土壤球囊霉素相关土壤蛋白质含量变异相比，第二代橡胶林各层次之间的土壤球囊霉素相关土壤蛋白质含量变异相对较小。橡胶林土壤有机质含量与土壤球囊霉素相关土壤蛋白质含量变化趋势并不一致。

关键词: 蚕豆, 蚕豆, 气象条件, 模糊数学理论, 隶属度

Abstract: Glomalin is an abundant soil glycoprotein, the soil total glomalin of different history rubber fields in hainan was extracted by 90 min of autoclaving (121 1C) 0.25 g soil in 2ml of 50mM sodium citrate adjusted to pH 8.0, and Bradford protein analysis was used for glomalin-related soil protein. The result showed that the concentrations of glomalin-related soil protein of rubber field soil in hainan baisha are relatively low, the effect of 8-years history of rubber planting on glomalin-related soil protein is not significant. The difference of glomalin-related soil protein concentrations of secondly rubber field layers is lower compare to that of glomalin-related soil protein concentrations of firstly rubber field layers. The trend of glomalin-related soil protein change is differ from soil organic carbon in rubber fields.