欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 85-88.

所属专题： 小麦

• 生物技术科学 • 上一篇    下一篇

航天搭载小麦种子SP3代的遗传变异分析

李鹏 张 锋,孙明柱,张凤云,高国强,李新华   

  • 收稿日期:2010-04-23 修回日期:2010-05-25 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    小麦航天工程育种技术创新与新品种创制;小麦空间环境诱变育种关键技术研究与示范;航天育种技术选育高产小麦新品种

Genetic Variation of Wheat SP3 Generation by Space Mutation

  • Received:2010-04-23 Revised:2010-05-25 Online:2010-12-05 Published:2010-12-05

PDF (PC)

1014

摘要：

为探讨航天搭载对小麦籽粒的诱变效应，给航天诱变育种提供理论支持，本研究利用酸性聚丙烯酰胺电泳和SRAP技术对航天搭载小麦品种白硬冬2号SP3 代种子的醇溶蛋白和基因组DNA进行了分析，结果表明航天诱变能够诱发醇溶蛋白和基因组DNA发生变异。在15个SP3代材料中，有8个材料的醇溶蛋白组成发生了变异，醇溶蛋白四个分区对航天诱变的敏感程度不同，以ω区最为敏感，产生的变异最多。利用25对SRAP引物对15个SP3代材料的基因组DNA变异进行了分析，共扩增出227条多态性条带，15个小麦航天诱变后代材料与对照间的遗传相似系数为0.821-0.937。

关键词: 草莓丰香, 草莓丰香, 灌溉模式, 产量, 根系, 光合特性, 水分利用率

Abstract:

In order to study the effects of space mutation of wheat, the gliadin and genomic DNA in the SP3 of Baiyingdong 2 were analyzed by acid polyacrylamide gel electrophoresis and SRAP markers, the results showed that the space-flight mutation could induce the variation of gliadin and genomic DNA. 8 mutants of gliadin were screened out from 15 SP3 materials, the induced variation effect also was different in four gliadin protein blocks，among that ω gliadin was the most sensitive and had the greatest variations. The genomic DNA variations of 15 SP3 materials were analyzed using 25 pairs of SRAP primers, and 227 polymorphic bands were amplified, genetic similarity coefficient between SP3 materials and control ranged from 0.821-0.937.