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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 81-84.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

水稻叶绿体的petG-trnP片段的测定和系统学分析*

刘志文   

  • 收稿日期:2010-08-09 修回日期:2010-09-25 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    第43批中国博士后科学基金项目;辽宁省教育厅项目

Study on the sequencing and phylogenetics analysis of the rice chloroplast DNA petG-trnP fragment

  • Received:2010-08-09 Revised:2010-09-25 Online:2010-12-05 Published:2010-12-05

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摘要：

本研究主要对水稻的叶绿体petG-trnP片段进行PCR直接测序和系统学分析。结果表明，水稻的petG-trnP的序列长度十分保守，其间隔区全长为316bp，扣除trnW序列后的非编码区长为242bp，其中两段非编码区分别为116bp和126bp。非编码区中G+C含量在37.19%-38.02%之间。对五种水稻材料进行ClustalW等分析表明，它们的序列结构有所变异，共有7个变异位点，比例为2.89%，这预示着水稻的petG-trnP的非编码区中有着一定的系统学信息，可在分子水平上为稻属的系统关系提供一定的证据。

关键词: 绿色廊道, 绿色廊道, 景观格局, 景观构成, 网络结构, 南昌市

Abstract:

The rice chloroplast petG-trnP fragment was selected to directly sequence and analyze in this study. The results demonstrated that the length of the rice petG-trnP intergenic spacer was very conservative and 316 bp with 242 bp non-coding region except the trnW sequence. The non-coding region was separated into two parts with 116bp and 126bp respectively. The G+C content was relatively low and about 37.19%-38.02% in the non-coding region. The 5 rice materials Clustal W analysis showed that the petG-trnP fragment structure was variable, there were 7 variable sites (2.89%) in the non-coding region. As a result, this indicted that it contained a few phylogenetic information and would provide the direct molecular evidence to clarify the Oryza species origin and evolution.