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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 31-36.

所属专题： 食用菌； 食用菌

• 生物技术科学 • 上一篇    下一篇

出口型香菇菌株亲缘关系分析及品比

姚强 任鹏飞 宫志远 高兴喜 刘岩 任海霞 曲玲 李瑾   

  • 收稿日期:2010-04-26 修回日期:2010-07-20 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    山东省自然科学基金项目：香菇基因组水平农杆菌介导的T-DNA插入突变;国家科技支撑计划项目（子课题）：秸秆栽培食用菌优良菌株选育;山东省农业良种工程项目：香菇转扩张蛋白基因的新品种选育

The Phylogenetic Relationship of Several Varieties for Export of Lentinula edodes and Comparative Experiment of Cultivation

  • Received:2010-04-26 Revised:2010-07-20 Online:2010-09-05 Published:2010-09-05

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摘要：

为了选育更适宜香菇菌棒出口的品种菌株，本文采用rDNA ITS序列分析了5个出口型香菇菌棒菌株的亲缘关系，并选择代表性菌株与国内几个主栽品种进行品比试验和国外适应性出菇栽培。结果表明，5个出口型香菇菌株具有一定的遗传多样性，可以分别聚为两个类群，其中菌株qh01、qh04、qh05和qh06聚为一组，说明这几个菌株具有较近的亲缘关系；而菌株qh02单独聚为一组，表明该菌株与其他几个菌株亲缘关系较远。从品比试验和适应性栽培试验结果来看，各菌株表现出明显的差异性，而其中2个用于菌棒出口的菌株qh02和qh06，无论是在生长速度、出菇产量及子实体性状上，还是国外适应性出菇栽培上，都明显优于其他菌株，表明这2个菌株是适合作为菌棒出口的菌株。

关键词: 重盐渍化土壤, 重盐渍化土壤, 改良, 灌水量, 改良剂

Abstract:

In order to select and breed new varieties of Lentinula edodes for export, the phylogenetic relationship of several strains of L. edodes was analyzed based on ribosomal DNA ITS sequences, and comparative experiments of cultivation between the two selected strains and some others cultivated in China were also investigated. The results indicated the moderate genetic diversity among these strains for export, and they could be clustered into two groups. The strains qh01, qh04, qh05 and qh06 were clustered into one group, which indicated a close phylogenetic relationship within the group. However, the single strain qh02 was clustered into another group, which inferred a far phylogenetic relationship with other strains. Comparative experiments showed various agronomic traits among the tested strains, and especially, the two strains qh02 and qh06, showed excellent agronomic traits, and consequently inferred that the two strains were suitable for export.