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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (23): 200-203.

所属专题： 生物技术； 烟草种植与生产

• 农学 农业基础科学 • 上一篇    下一篇

Atfad8叶绿体转基因烟草对冷胁迫的生理响应

张梦 高 岩 李薇薇 郭东林 郭长虹   

  • 收稿日期:2010-04-30 修回日期:2010-08-26 出版日期:2010-12-05 发布日期:2010-12-05
  • 基金资助:

    国家自然科学基金;教育部博士学科点专项科研基金;黑龙江省国际合作项目基金;哈尔滨师范大学青年骨干教师科研项目基金

Physiological Responses to Chilling Stress in Transplastomic Tobacco with Atfad8 Gene

  • Received:2010-04-30 Revised:2010-08-26 Online:2010-12-05 Published:2010-12-05

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摘要：

摘 要：采用5℃低温处理，研究Atfad8叶绿体转基因烟草对冷胁迫的生理响应。结果表明:随着低温胁迫时间的延长，烟草幼苗的丙二醛(MDA)含量和过氧化物酶(POD)活性呈增加趋势，超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性先上升后下降。 Atfad8叶绿体转基因烟草的MDA含量明显低于对照植株，而抗氧化酶活性明显高于对照植株。Atfad8基因的导入提高了烟草幼苗的耐冷害能力。

关键词: 番茄, 番茄, 农业废弃物, 基质, 工厂化育苗, 壮苗指数

Abstract:

Abstract: Physiological response to cold stress in transplastomic Atfad8 tobacco plants and non-transgenic tobacco plants were investigated at 5℃ temperature. The result showed that the content of Malonaldehyde (MDA) and activity of POD under 5℃ treatments were increased, while the activity of SOD and CAT were decreased with the time. Under the cold stress, the activities of POD, SOD and CAT in transplastomic tobacco plants were obviously higher than those in the non-transgenic tobacco plants. The contents of MDA in transplastomic tobacco plants were obviously lower than those in the non-transgenic tobacco plants. The results showed that over expressing Atfad8 gene in chloroplast genome improved the chlling resistance ability of plants.