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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (20): 335-338.

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

一株黑曲霉转化低品位磷尾矿能力的研究

李露莉 邱树毅 王义娟 何贵香   

  • 收稿日期:2010-05-25 修回日期:2010-07-02 出版日期:2010-10-20 发布日期:2010-10-20
  • 基金资助:

    磷复肥固废物利用及治理石漠化技术研究

Reaserch on the Transforming Capacity of Low Grade Phosphate Tailings with Aspergillus niger

  • Received:2010-05-25 Revised:2010-07-02 Online:2010-10-20 Published:2010-10-20

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摘要：

研究了在液体纯培养条件下，低品位磷尾矿粉用量、不同氮源和pH值对黑曲霉（Aspergillus niger）溶磷活性的影响，并初步探讨了其溶磷机制。结果表明，A. niger产生的有机酸主要是草酸，它在尾矿粉的溶解浸磷过程中起重要作用。磷尾矿粉的用量为5 g/L时，最大溶磷量是56.02 mg/L，溶磷率高达20.87%；磷尾矿粉的用量超过5 g/L时，溶磷能力显著降低，溶磷率由5 g/L时的20.87%降至40 g/L时的0.65%。此外，以(NH4)2SO4作为氮源时，最大溶磷率是以NaNO3为氮源时的2.17倍。pH值在5~6之间变化时，溶磷率达到最高。

关键词: 灵芝, 灵芝, 孢子, 萌发, 影响因素, 萌发率

Abstract:

The effect of low grade phosphate tailings (LGPT) concentration, different nitrogen source and pH in the media on phosphate solubilizing activity of Aspergillus niger was studied under liquid pure culture medium, and the mechanism of phosphate solubilization was explored. The result showed that oxatic acid which plays an more important role in the dissolution of LGPT is the main organic acid produced by A. niger in the culture peroid. When 5 g LGPT was added into one liter of the medium, the maximum phosphate solubilization capacity and phosphate solubilization efficiency are 56.02 mg/L and 20.87%, respectively. When more than 5 g LGPT was added into one liter of the medium, the phosphate solubilization efficiency decreased from 20.87% (5 g/L LGPT) to 0.65% (40 g/L LGPT). Moreover, when (NH4)2SO4 was supplied as nitrogen source, the maximum phosphate solubilization efficiency are 2.17 times higher than that of NaNO3. Besides, there is the highest phosphate solubilization efficiency when pH value is from 5 to 6.