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中国农学通报 ›› 2011, Vol. 27 ›› Issue (4): 173-177.

• 林学 园艺 园林 • 上一篇    下一篇

ISSR分子标记技术在桃品种鉴定中的应用

孙淑霞 李靖 陈栋 谢红江 涂美艳 江国良   

  • 收稿日期:2010-07-06 修回日期:2010-09-25 出版日期:2011-02-10 发布日期:2011-02-10
  • 基金资助:

    桃公益性行业;成都市技术应用与推广计划重点项目

Molecular Identification of Peach Germplasm by ISSR Markers

  • Received:2010-07-06 Revised:2010-09-25 Online:2011-02-10 Published:2011-02-10

摘要:

为了有效地鉴别桃苗木优良品种或类型,为桃种质资源研究和新品种保护提供理论基础,利用ISSR标记对28份桃种质进行了品种鉴定及亲缘关系检测。结果发现:23条ISSR引物共扩增出188条DNA片段,其中96条具有多态性,多态性频率为51.06%;材料间遗传相似系数GS变幅为0.84~0.97,平均为0.92。获得28份供试材料的指纹图谱,鉴定出9条特异出现DNA片段和11条特异缺失DNA片段。通过UPGMA聚类分析发现,供试材料中地理来源及部分农艺性状相近的品种或类型聚为小类,但大的聚类群没有明确的划分标识,可能与桃种质在地区间互换及所选择的ISSR标记有关。

关键词: 科技, 科技, 农业产业化, 技术链, 战略

Abstract:

To differentiate and identify varieties and types of peach germplasm, the genetic diversity and cultivar identification among 28 peach accessions by ISSR markers were explored. Twenty three ISSR primers amplified 188 bands, 96 bands were polymorphic, and the polymorphic percentage was 51.06%. The genetic similarity coefficient (GS) estimated from these molecular data ranged from 0.84 to 0.97, with an average of 0.92. Each genotype had a unique banding profile, and a total of 20 specific ISSR fragments were available to be converted into sequence characterized amplified region (SCAR) or simple sequence repeat (SSR) markers. UPGMA analysis showed that there were not distinct clusters and sub-clusters were consistent with geographical origins and agricultural character of peaches. The results of cluster analysis might be related to the germplasm exchanges between different regions and the characteristics of ISSR markers.