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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 34-37.

所属专题： 水稻

• 农学 农业基础科学 • 上一篇    下一篇

陕西汉中地区主栽水稻品种的SSR多态性分析

张羽 陈雪燕 王胜宝 莫丹   

  • 收稿日期:2010-08-12 修回日期:2010-09-03 出版日期:2011-04-05 发布日期:2011-04-05
  • 基金资助:

    陕西省自然科学基础研究计划项目;陕西省教育厅专项科研项目

Analysis of SSR Marker-based Polymorphism of Rice Mainly Popularized in Hanzhong

  • Received:2010-08-12 Revised:2010-09-03 Online:2011-04-05 Published:2011-04-05

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摘要：

掌握陕西省汉中地区主栽水稻品种的遗传多样性，了解其遗传背景，明确各品种间的亲缘关系。从水稻12条染色体上的30个SSR标记中筛选出14对有效引物，对陕西省汉中地区20个主要种植的水稻品种的遗传多样性进行分析。结果共检测到69个等位基因，平均每个标记检测到5个等位基因，每个SSR位点的遗传多态性信息含量在0.27~0.74之间，平均值为0.48。聚类分析表明，20个水稻品种的遗传相似系数集中在0.55~0.94之间，遗传相似度较高，亲缘关系较近。笔者认为，SSR标记所作的品种聚类分析与系谱法趋势一致，汉中地区水稻种质资源遗传基础相近是其水稻产量和品质难以突破的原因之一。

关键词: 种质资源, 种质资源, 核心种质, 取样策略

Abstract:

Mastering genetic polymorphism of rice varieties mainly popularized in Hanzhong, understanding their genetic background, clearing the relationship between all varieties. The genetic diversity of 20 rice varieties in Hanzhong, Shanxi Province was investigated with the technique of SSR. By using 14 effective primers, which were screened from 30 SSR primers distributed on all the 12 rice chromosomes. The results showed that: 69 alleles were detected by 14 pairs of SSR primers. The average number of alleles per SSR locus was 5. The average number of polymorphism information content per SSR primer was 0.48 with a range from 0.27 to 0.74. Genetic similarities ranged from 0.55 to 0.94. The genetic similarity was high and the genetic relationship was closed. The author thinks that the clustering analysis based on SSR marker and trend of pedigree method are fit. and it is one of the reasons that the yield and quality on rice is not easy to break because of near genetic basis of germplasm in Hanzhong.