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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 46-49.

所属专题： 小麦

• 农学 农业基础科学 • 上一篇    下一篇

小麦-黑麦代换系间杂交后代的SSR分析

丁海燕 张利敏 郑茂波 李集临   

  • 收稿日期:2010-08-19 修回日期:2010-09-23 出版日期:2011-04-05 发布日期:2011-04-05
  • 基金资助:

    国家自然科学基金（No.39670417）

Analysis on the Offsprings from Crossing between Wheat-Rye Substitution Lines by SSR

  • Received:2010-08-19 Revised:2010-09-23 Online:2011-04-05 Published:2011-04-05

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摘要：

为了深入了解7个大穗型小麦品系的遗传基础及更好地利用其对小麦进行遗传改良，利用SSR技术，对从小麦-黑麦代换系5R/5A与6R/6A杂交后代中选育的大穗型品系08-4、08-5、08-6、08-7、08-8、08-9、08-12进行了分析。结果表明，使用位于黑麦染色体上的17个SSR引物，在15个引物位点上，7个品系带型同普通小麦的相似。从所用引物中筛选出引物Xgwm247-6RL，在08-4、08-8、08-12和黑麦中扩增出约168bp、178bp、190bp 的三条黑麦特异片段，证明了品系08-4、08-8、08-12 中导入了黑麦6R染色体长臂的遗传物质。由于这些品系具有大穗、多小穗等优良性状,是有经济价值和利用价值的遗传材料。

关键词: 杏, 杏, 果实, RNA, 提取, RT-PCR

Abstract:

In order to study their genetic component and use them better, seven lines, 08-4、08-5、08-6、08-7、08-8、08-9、08-12,with big ears which came from crossing between wheat-rye substitution line 5R/5A and 6R/6A were identified by SSR.The results are followed: 17 SSR primer pairs located on chromosomes of rye were used for SSR amplification.The specific DNA fragments of rye, 168bp、178bp、190bp ,can be amplified by pimer Xgwm247-6RL in the lines 08-4、08-8、08-12. These results indicated that lines ,08-4、08-8、08-12, contained rye chromatin of 6RL. Because they have big ears and multispikelets with more number of grains per ear, these lines have very important value in wheat breeding and genetics improvement .