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中国农学通报 ›› 2011, Vol. 27 ›› Issue (3): 215-220.

• 农学 农业基础科学 • 上一篇    下一篇

纤维素产氢菌的分离及不同秸秆处理方法的研究

刘冰 王爱杰 韩梅   

  • 收稿日期:2010-10-14 修回日期:2010-11-05 出版日期:2011-02-05 发布日期:2011-02-05

Isolation of Cellulose Bacteria Producing Hydrogen and the Study of Straw Pretreatments

  • Received:2010-10-14 Revised:2010-11-05 Online:2011-02-05 Published:2011-02-05

摘要:

为了增强生物方法对秸秆的利用能力,将秸秆较好地转化成氢能,本试验利用改良的Hungate厌氧滚管法及滤纸崩解法,从已得菌系JYB中,筛选得到1株能较好地降解纤维素产氢的细菌,并利用水热法、汽爆法等6种方法对秸秆进行预处理,以增强秸秆的产氢效果。结果表明:筛选得到菌株JYB-13,该菌可利用大量碳源产氢,产氢的最适条件为35℃,pH值8.5。对秸秆进行预处理,由产氢效果得知,该菌对2%硫酸处理后的秸秆降解效果最好,秸秆的降解率为53.1%,氢气含量达到37.2%。分析发酵前后秸秆的成分,可知该菌可以较好地利用纤维素和半纤维素,对木质素利用较少。

关键词: 洋葱, 洋葱, 产业, 回顾, 展望

Abstract:

In order to strengthen the effect of degradating straw and turn straw to hydrogen, the author used a modified Hungate anaerobic roll tube method and the disintegration of filter paper method, found a strain which could have better hydrogen production by degradating cellulose, and used 6 kinds of method pretreatments, such as hydrothermal method, the vapor on straw stem exploding and so on to treat straw. The author found a bacterium JYB-13 from already getting strains JYB, JYB-13 could produce hydrogen using amount of carbon sources. Its optimum conditions for hydrogen production temperature of 35℃, pH value of 8.5. Using 6 kinds of method pretreatments, by the effect of producing hydrogen, it was concluded that the strain in 2% sulfuric acid treated straw degradation was the best. After fermentation, the degradation rate of straw was 53.1%, the hydrogen content reached 37.2%. Around the analysis of the composition of straw obtained before and after fermentation, JYB-13 could good use of cellulose and hemicellulose better, and few used lignin.

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