Abstract: The sterile lines with Ae.kotschyi cytoplasm (shortened:K-type) and their corresponding preserve lines, restoring lines and hybrids were used in this paper to study the difference in seed germination and seedling emergence between Ae.kotschyi cytoplasm and T.aestivum cytoplasm. The results showed that there was no significant difference between the two cytoplasm in seed germination and seedling emergence of 3cm planting depth, but that it was different with different nuclear background in seedling emergence of 6cm planting depth —which of the sterile line Yumai No. 3 and its hybrid was a little higher, and which of the sterile line S43 and its hybrid was significantly lower.