INTERSPECIES NUCLEAR TRANSPLANTATION BETWEEN MOUSE AND RABBIT

Abstract

Abstract: When mouse 2~8Cell stage embryo blastomeres used as nuclear donor, and rabbitoocytes 15h、17h past hCG used as cytoplasma recipient , the proper fuse and activation parameter of hybrid embryos were two DC pluse of μs 10 min interval in 0.3 mol/L manitol solution contain Ca2+、Mg2+. The fuse rate of moude blastomeres from 2 or 4—Cell Stage embryos and enucleated rabbit oocytes were higher thawn those from 8—Cell stage embryos (83%,80%vs.62.5%) ,the morulae and blastocysts yate were no significantly difference, Co—culture with somatic cell and Taurine could significantly improve the developmental awbility of mouse-rabbit nucleo-cytoplawsmic hybrid embryos .

Wang Xinzhuang， Zhang Defang Zhang Yong Li Yuqiang Tian Wanqiang,. INTERSPECIES NUCLEAR TRANSPLANTATION BETWEEN MOUSE AND RABBIT[J]. Chinese Agricultural Science Bulletin, 2004, 20(5): 4-4.

References

1 McGrath J，& Solter D. Nuclear transplantation in mouse embryos. J Exp Zool. 1983,228:355~362
2 Solter D. Dolly is a Clone-and no longer alone. Nature .1998,394:315~316
3 Yang X, Zhang L, Kovacs A, Tobback C， et al . Potential of hypertonic medium treatment for embryo mictomanipulation I: assessment of nuclear transplantation methodology.isolation,subzona insertion and electrofusion of blastomere to intact or functionally enucleated oocytes. Mol Reprod Dev. 1990,27:118~129
4 Collas P , Robl J M. Development of rabbit nuclear transplant embryos from morula and blastocyts stage donor nuclei. Theriogenology. 1991,35(1):190
5 Collas P, Pinto-Correla C, Ponce de leon F A， et al. Effect of donor cell cycle stage on chromatin and slindle morphology in nuclear transplant rabbit embryos. Biol Reprod. 1992,46:501~511
6 Robl J M, Gillgan B, Crister E S ，et al. Nuclear transplantation in mouse embryos: assessment of recipient cell stage. Biol Reprod. 1986,34:733~739
7 Collas P, Balise J J, Robl J M. Influence of cell cycle stage of the donor nucleus on development of nuclear transplant rabbit embryos. Biol Reprod. 1992,46:492~500
8 Collas P ， Barrnes F L. Nuclear transplantation by microinjection of inner cell mass and granulosa cell nuclei. Mol Reprod. 1994,38:264~267
9 Robl J M, Prather R, Eyestone W， et al. Nuclear transplantation in bovine embryos. Theriogenology. 1986,25(1):189
10 Tanaka H , Kanagawa H . Influence of combined activation treaments on the success of bovine nuclear transfer using young or aged oocytes. Anim Reprod Sci. 1997,49 (2):113~123
11 Prather R S, Barnes F L, Sims M M， et al. Nuclear transplantation in the bovine embryos:assessment of donor nuclei and recipient oocytes. Biol Reprod. 1987,37:859~866
12 Sims M ，First N L. Production of fetuses from totipotent cultured bovine inner cell mass cells. Theriogenology. 1994,39:313
13 Bondioli K R. Nuclear transfer in cattle. Proc. Intl. Sympo. Anim. Biotech, Kyoto. Japan. 1991.10
14 Cambell KHS,Ritchie W A ,Wilmut I. Nuclear-cytopasmic interactions during the frist cell cycle of nuclear transfer reconstructed bovine embryos: implications for Deoxyribonucleic acid replication and development. Biol Reprod. 1993,49:933~942