Abstract: The absorption spectrums and extraction efficiencies of chlorophyll from maize seedling after frozen treatment were compared on six determined methods using different organic solvents. The results showed that the absorption spectrums of five solutions were identical with that of acetone solution, so Arnon’s formula of chlorophyll was suitable for other methods. In house temperature, soaking methods were better than Arnon method. The extraction efficiencies of chlorophyll using mixture solution better than using acetone or ethanol alone, methods of acetone mixed ethanol were preferable for fast extraction and stability. Soaking methods in 50℃ after frozen treatment could accelerate extraction of chlorophyll, but also speed up degradation of chlorophyll, so light screening was necessary.