Abstract: In order to learn soil fertility of main district planting flue-cured tobacco and give some reasonable advice on scientific fertilization, 384 soil samples from 8 main districts planting flue-cured tobacco were analyzed. The results showed that nitrogen of every district planting flue-cured tobacco was rich. NH4+ nitrogen of Tongren was richest. It was 21.41mg/kg. One of Guiyang was lowest. It was 17.06 mg/kg. NO3- nitrogen of Guiyang was richest. It was 23.47 mg/kg. One of Tongren was lowest. It was 17.99 mg/kg. Available phosphor of Qiannan was richest. It was 18.07 mg/kg. One of Qianxinan was lowest. It was 8.29 mg/kg. Available potassium of Zunyi was richest. It was 178.29 mg/kg. One of Qiandongnan was lowest. It was 137.36 mg/kg. Organic matter of Liupanshui was richest. It was 34.64g/kg. One of Tongren was lowest. It was 22.53 g/kg. The value of pH of every district planting flue-cured tobacco was under of 7.Generally compared, soil fertility of Zunyi, Bijie and Qiannan was best and it was good condition could cultivate high quality flue-cured tobacco.