Abstract: 【OBJECTIVE】 Studied the effects of different applying combination of biogas fertilizer on soil fertility and the quantity of microbes, the fertilizing method through base fertilizer and top fertilizer. 【METHOD】 Five plots of each district , three repeats, a randomized complete block design, the fertilizing method including base fertilizer and top fertilizer, two stages facilities were the same【RESULTS】 The results showed that using biogas fertilizer and biogas fertilizer with bacterial manure could increase the contents of N、P、K , volume weight of soil had tendency of dropping by only using biogas fertilizer, using biogas fertilizer with bacterial manure, using biogas fertilizer with bacterial manure and fertilizer ;The quantities of virus, ray fungi and leaf mold had increased when used biogas fertilizer , it better to use biogas fertilizer with bacterial manure【CONCLUSION】biogas fertilizer with bacterial, biogas fertilizer with fertilizer and bacterial and only biogas fertilizer could significantly reduce soil bulk density, improve soil structure, increase the volume of virus, ray fungi and leaf mold.