Abstract: 【Objective】To clone and analysis the sheep intelectin . 【Method】Total RNA was extracted from duodenal mucosa tissue and mRNA sequence of gene were amplified by RT-PCR using designed primers, the upstream of which was designed from the contig by silico cloning, the downstream of which was designed in conservative region. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of E. coli JM109.The sequence was analyzed to identify the recombinant plasmid. 【Results】Identity analysis showed that the intelectin nucleotide sequence shared 93.3%, 87.1% ,82.9% and 78.6% homology with that of bovine (predicted), porcine , human and rat. The predicted peptide contained a FBG motif. 【Conclusion】 The sheep intelectin-2 was successfully cloned and the sequence has been submitted to GenBank(Accession. EF624459).