Abstract:

【Abstract】Aim：In the present study, we observed the effects of bufonis parenteral solution on in vitro immunocytes proliferation and three kind of cytokines secretion. Methods：We choose the traditional Chinese immunopharmacology method to test the effect of bufonis parenteral solution on the immunocytes. We take the Balb/c mouse spleen lymphocyte incubate with bufonis parenteral solution combine with LPS or Con A, for test spleen proliferation activity; bufonis pareteral solution incubate with peritoneal macrophage for test the capability of phagocytosis; bufonis pareteral solution incubate with NK cell, for test the cytotoxic activity of Yac-1 cells. We also evaluated spleen lymphocyte secrete cytokines by ELISA method. The results showed that bufonis parenteral solution treated alone or combined with polyclonal T cell mitogen (Con A or LPS) in a certain dose range, the effects significantly enhanced the proliferation, and the solution significantly promote the PMΦ phagocytosis neutral red capacity (P<0.05, P< 0.01). Bufonis parenteral solution alone significantly enhanced the NK cytotoxic activity of Yac-1 cells (P<0.05, P<0.01). Bufonis parenteral solution also induce cytokines secretion in vitro (P<0.05, P<0.01). The results indicated that bufonis parenteral solution not only enhances adaptive immunity, but also activates innate immunity.