Abstract:

The cotyledons and hypocotyls explants from Zhaodong alfalfa and Pleven6 alfalfa ，the establishment of in vitro regeneration system. The results are as follows : the best callus induction medium of Zhaodong alfalfa was MS +2 .0mg/L2,4-D +0.5 mg / LKT +3% sucrose and of Pleven6 was MS +1.5 mg/L2,4-D +0.5 mg / LKT +3% sucrose; Differentiation medium: MS +2.0 mg/L6-BA +0.5 mg / LNAA +3% sucrose; Differentiation subculture medium: MS +0.3 mg/L6-BA +0.1 mg / LNAA +0.3 mg / LKT +3% sucrose; Induced rooting medium: 1/2MS +0.5 mg / LNAA +1.5% sucrose. At the same level of the experiment, induction rate and differentiation rate of Zhaodong alfalfa was significantly higher than pleven6 alfalfa, and both cotyledons used shorter differentiation time and higher differentiation rate than hypocotyls.