Abstract:

Objective: we present a method based on liquid chromatography tandem mass spectrometry for determining residues of diclazuril in chicken tissues and investigate the depletion of diclazuril in chicken tissues. Method: The analyte was extracted by mixed solution of acetonitrile and ethyl-acetate, The extracts were dried by nitrogen gas, redissolved in acetonitrile, filtered through 0.22μm micropore film and directly injected into the LC–MS/MS system. Result: The calibration curves were linear (r2>0.99) in the range of 8~4000μg/kg in chicken liver and 8~1200μg/kg in chicken muscle. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.5μg/kg and 0.8μg/kg. Conclusion: The method was simple and accurate enough to determine residues of diclazuril in chicken tissues.