Abstract: To find the influential factors for inducement of Acanthopanax senticosus hairy roots, this test used the three Agrobacterium tumsfeciens R15834, R1601 and C58C1 as test bacterium, leaf, leafstalk and stem as explants, to study different concentration of bacterium, different time of co-cultivation and Acetosyringo (As) how to affect the root hairs transformation frequencies of Acanthopanax senticosus. The most appropriate OD600 of R15834 suspension was about 0.6. Young leaves as explant were more suitable experimental material. The most appropriate co-culture time was about 4 days. As concentration was 400 μmol/L, the induction rate was highest. This research has established the foundation on genetic improvement of Acanthopanax senticosus