Abstract:

The polysaccharides and polyphenols in Acer palmatum leaves have seriously affected the purity and quality of RNA extraction. In order to obtain the appropriate method of total RNA extraction, three methods of column plant RNAout, TRIzol reagent and improved CTAB were chosen for comparison analysis of total RNA extraction from Acer palmatum. Total RNA extracted by column plant RNAout method had the highest yield, but the worst quality due to the pollution of DNA. The worst quality of total RNA by TRIzol method was due to the lowest yield and the pollution of carbohydrate and phenols. By use of the improved CTAB method, the ration value of OD260∕OD280 of total extracted RNA was 2.0, as well its clear electrophoresis pattern showing the good quality, high purity and higher yield. So the improved CTAB method is suitable for the total RNA isolation from the leaf of maple. The total RNA was used for RT-PCR and the specific band could be observed in agarose gel. These results demonstrate that the quality and purity of the RNA obtained by the improved CTAB method can meet the demands of molecular biology experiment. The improved CTAB method was the appropriate method of extraction the total RNA in Acer palmatum leaves.