Abstract:

In order to ascertain the cultivated conditions and the optimal medial components of Bacillus subtilis B-332, the cultivated conditions for Bacillus subtilis B-332 were optimized by single factor and orthogonal experimental designs. The results showed that the optimal seed aged 18 h, and the optimal fermentation time was 72 h, the optimal medial components were composed of 0.60 g/L soybean oil meal, 0.25 g/L saccharose, 0.07 g/L ammonium sulfate, 0.03 g/L trisodium citrate, 0.003 g/L dipotassium phosphate, 0.005 g/L magnesium sulfate, 0.0005 g/L ferrisulphas. Under these conditions, numuber of spores was 1.43×1011 cfu/mL, and increased 34.48 times compared to initialization, and it formed the base for the factory.