Abstract:

In the present study, researchers observed the regulating action of Venenum Bufonis injection on Newcastale Disease（ND） and Infectious Bronchitis（IB） live vaccine. Methods: Researchers choose the traditional Chinese immunopharmacology method and combined different concentration Venenum Bufonis injection and ND and IB live vaccine as test groups. On the 7th, 14th, 21th, 28th, 35th, 42th, 49th and 56th day after immunization, researchers took blood samples through vein and choose the MTT method to test the optical density(OD) of peripheral blood T cells and B cells. Researchers took β-micromethod to test the antibodytiter of ND and IB in blood serum. The results showed that in the all of the test groups, Venenum Bufonis injection can observably increase OD of peripheral blood T and B cells. In the medium dosage group (0.25mg/mL), the OD of T cells and B cells was the highest. Venenum Bufonis injection can significantly increase the antibodytiter of ND and IB in blood serum. In the medium dosage group (0.25mg/mL), antibody existence lasted longer in late immunization and antibody peak hour lasted longer too. Therefore, Venenum Bufonis injection is the effective adjuvant of ND and IB live vaccine.