Abstract:

The outer membrane of the Riemerella Anatipestifer (abbreviate as RA )are extracted through Sds method,Sarkosyl method,PMSF method,and are electrophoresized repectively.test result showed that three methods can extract the outer membrane of RA,but display differently electrophoresis bands by Coomassie brilliant stained；by comparison,Sds method is most suitable for extracting the outer membrane of RA; whether or other proteins whose molecular weight are different by the three methods belong the outer membrane protein antigeon,which need to be research farther more.