Abstract:

In order to clarify the processes and mechanisms of the biosynthesis of natural rubber, based on EST sequence from an SSH cDNA library of Hevea brasiliensis, a full-lengh cDNA encoding Calmodulin,designated HbCAM1,was cloned from H.brasiliensis by RACE-PCR, which has a total length of 775 bp with an open reading frame (ORF) of 450 bp and encodes 149 amino acid residues. The deduced amino acid sequence showed high identity of 100%,100%,99% and 99% to those of CAM from Ricinus communis，Vitis quinquangularis, Nicotiana attenuate and Jatropha curcas.Semi-quantitative RT-PCR analysis indicated that the transcription of HbCAM1 in latex was induced by tapping and ethphon treatment，suggesting that HbCAM1 may be involved in the regulation of ehphon-induced high latex yield in Hevea brasiliensis.