Abstract:

BADH (betaine-aldyhyde dehydrogenase, EC 1.2.1.8) cDNA was amplified by PCR, including XhoI (upstream) and SacI (downstream) restriction sites respectively. This BADH fragment was cloned into the vector pTA2 (obtaining pTA2-BADH) and verified by sequencing. Then the fragment was cloned from recombinant plasmid pTA2-BADH into the vector pBA002 by digestion and ligation. The obtained pBA002-BADH vector was further verified by enzyme digestion, PCR amplification, and sequencing. Using the plant expression vector pBA002-BADH, we are able to conduct further plant genetic transformation.