Abstract:

In order to obtain wheat wx-B1a gene-specific RNAi expression vector，the total RNA was isolated from wheat grain of 12-day post anthering, a pair of special primers was designed according to the wx-B1a gene (GenBank No. AB019623), wx-B1a partial cDNA sequences (461 bp) was amplified by RT-PCR. The results demonstrated that, the cloned wx-B1a gene sequences were high homology with the reported waxy genes in GenBank previously. Then the fragment ligated to the FAD2 Intron1 (Arabidopsis FAD2 Intron) in sense and antisense orientation to produce a hairpin fragment. The hairpin fragment was then inserted downstream of the endosperm specific expression promoter of high moleculer weight glutein gene 1Dx5. And a RNAi vector was obtained, which was drove by HMW-GS 1Dx5 promoter in pBAC47p. This RNAi vector will be used to breed good-quality noodle lines with high yield-strong gluten wheat.