Abstract:

The aim was to study the linkage and genetic mechanisms of nuclear male sterile in rice. In this study, pollen fertility and seed setting observation were used to investigate in exploring crossing with three male varieties by bulk segregant analysis of F2 population between the parents and two bulks population representing fertile and sterile plants. The results indicated that male-sterile gene of ‘Lu18S’ belonged to one recessive nucleus gene inheritance by one RAPD primer S490 amplified a length of 850 bp fragments. It showed close linkage with sterile gene. The length of the fragment was coincided with Kotb’s result. The RAPD linkage markers were obtained. It provided the basis for the study of sterile gene clone and location.