Abstract:

In order to identify hybrids timely and accurately to increase select the efficiency of breeding, four isozymes (EST, SKD, MDH, 6-PGD) in forty progenies of eleven lily hybrid combinations were analyzed by polyacrylamide vertical plate gel electrophoresis. The results showed that: (1) With the 40 progenies, 30 samples were true hybrids while others could not be identified; (2) Among EST, SKD, MDH and 6-PGD bands, the combination number of male parent has feature bands compare with female parent were 8,2,0,0 respectively; (3) The combination 2 and 3 was reciprocal cross, it could not find specific genetic; (4) The EST bands were varied at different stages. Because of base and feature bands were stabled, the differences didn’t affect the result. In a word, isozyme could be considered as a mean to identify hybrids. In the isozyme analysis, EST and SKD play significant roles.