Abstract:

The aim was to establish SRAP-PCR amplification system which was suitable for Chinese Bayberry genome DNA. The optimized SRAP-PCR amplification system for Chinese bayberry was established by the orthogonal design. The results suggested that the order of factors which affect on the result of SRAP-PCR were Mg2+, template DNA, dNTPs, primers and Taq DNA polymerase. A suitable SRAP-PCR system for Chinese bayberry was that total 25 μL reaction system containing 2.5 mmol/L Mg2+, 50 ng template DNA, 0.25 mmol/L dNTPs, 0.15 μmol/L primers and 1.5 U Taq DNA polymerase could be able to amplified the most rich polymorphism and clear bands. The optimized SRAP-PCR system was tested on twelve Chinese bayberry germplasms and shown to be steady and reliable for molecular genetics research.