Abstract:

In order to explore best extraction process and insecticidal activities of limonin from Citrus maxima peel, the extraction technology of limonin from Citrus maxima peel was optimized through orthogonal test, and studied insecticidal activities of the limonin to Aphis nerii, Lcerya purchasi and Eurydema gebleri. The results showed that: 0.9700 g, 1.5860 g and 1.6000 g limonin were obtained from 10 g through ultrasonic extraction, methylene chloride and acetone water bath extraction, extraction efficiency was 6.08% of limonin in Citrus maxima peel through methylene chloride water bath extraction. 10 mg/mL limonin would kill all the pests tested, 0.625 mg/mL limonin still had higher insecticidal activity. The dichloromethane was best extractant for extracting limonin from Citrus maxima peel and the optimal extracting technological conditions were as follow: the ratio of solid to liquid at 1:12, refluxed for 2 times and 2 h each time. There was strong insecticidal activity of limonin in Citrus maxima to 3 kinds of the insect pests tested.