Abstract:

In order to study the mechanism of bioremediation in fomesafen-contaminated soil, a fomesafen degradable strain was obtained from fomesafen -contaminated soil. F-12，a bacterium which could use fomesafen as sole carbon source for growth, was isolated from the soil of long term applied with fomesafen by enrichment culture. Base on colony morphology, physiological and biochemical characteristics and 16SrDNA gene sequence analysis, the strain F-12 was identified preliminarily as Klebsiella sp.. The effect of initial concentration of fomesafen, inoculation amount, temperature and pH on degradation efficiencies was studied. The optimal degrading conditions were: initial concentration of fomesafen 100 mg/L, inoculation amount of 15%, pH 6.0, respectively, under the optimal conditions, the degrading efficiency could reach more than 80% after2 days at 35℃, holding the potential of being applied in the fomesafen bioremediation of contaminated soils.