Abstract:

The aims were to clone a full-length cDNA (MrActin1) of actin from Myrica rubra leaves, and evaluate its evolution status and analyze the expression model of MrActin1. A full-length cDNA of actin was obtained using RT-PCR techniques. The physicochemical properties and homology analyzed by bioinformatics methods. The phylogenetic tree of actin family was constructed by neighbor-joining. The expression model of MrActin1 investigated in various organs of Myrical rubra. The full length of MrActin1 was 1137 bp (GenBank accession No. AB 650589), encoding a protein of 377 amino acids. Homologous alignment showed that it shared over 88% nucleotide sequence similarity. The phylogenetic tree reconstructed on the base of amino acid sequences suggested that the relationship of actin between Myrica rubra and Gossypium hirsutum, Malva pusilla was most intimate and they might have the same differential time in evolution. The analysis by Northern blot showed that MrActin1 was constantly expressed in various organs of Myrical rubra such as flowers, leaves, branches, fruits. The full-length cDNA sequence of MrActin1 was firstly obtained.