Abstract:

Improving the extraction efficiency of flavonoids from plumula nelumbinis, pectinase assisted extraction has been adopted. The effects of enzymatic hydrolysis temperature, pH value, enzyme dosage and enzymatic hydrolysis time on extraction yield of flavonoids from plumula nelumbinis were investigated. The processing parameters were optimized by orthogonal array design. The results showed that the order of the primary and secondary factors for the yield of flavonoids was enzyme dosage>enzymatic hydrolysis temperature>enzymatic hydrolysis time>pH value. The optimal extraction conditions were enzymatic hydrolysis temperature 50℃, pH 4.5, enzyme dosage 0.6 mg/mL, and enzymatic hydrolysis time 2.0 h, the extraction yield of flavonoids from plumula nelumbinis was 1.271% on optimum technological conditions. Compared to traditional water extraction method (90℃、45 min), the pectinase assisted extraction method has the advantages of improving yield with lower extraction temperature. The extraction yield of flavonoids from plumula nelumbinis has increased by 28.64%.