Abstract:

In order to solve the soil chloride-ethyl residue on the soil of hazards, from the long-term application of chlorimuron-ethyl in soil isolated one pair of chlorimuron-ethyl with degradation of the strain. Through the use of physiological and biochemical, 16S rDNA identification and high-performance liquid chromatography was to study the physiological and biochemical characteristics, and degradation characteristics. The results showed that, the strain of Pseudomonas, the optimum growth temperature of 30-35℃, pH 5-6, the best inoculum size was 5%. Ultimately concluded that, the optimum growth conditions in bacteria could be 100 mg/L of ethyl chloride as the sole nitrogen source in the medium, and cultured for 5 days, the degradation rate of 80%, mixed with its L-7 degradation rate to 90%.