Abstract:

To find new resistance genes and study perilipin gene 8-3 related to Sclerotinia sclerotiorum, a couple of primers containing restriction enzyme sites of Xba I and BamH I were designed to clone the 8-3 from cloning vector pMD19-T-8-3, and the product was digested and lignased with plant expression vector pBI121. The pBI121-8-3 was transformed into LBA4404 and the 8-3 gene was transformed into Nicotiana tabacum 89 mediated by Agrobacterium tumefaciens. 35 resistant lines were obtained, 21 of which were identified as transgenic plants by PCR and sequencing. The further identification of RT-PCR was confirmed that the 8-3 was expressed in the transgenic plants. The transgenic tobacco infected with Sclerotinia sclerotiorum and oxalic acid showed that 8-3 could improve the plants' tolerance level to oxalic acid and enhance the resistance to Sclerotinia sclerotiorum to a certain extent, which laid the foundation for the study of 8-3 gene and plant resistance to Sclerotinia sclerotiorum.