Abstract:

The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. The results showed that all strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of a factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mating types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isoenzyme test. Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing mating genotype analysis with esterase isozyme analysis, it showed great similarity. Mating genotype analysis could be an important supplementary method to strains identification and genetic diversity research.