Abstract:

The aim was to obtain the photosynthetic bacteria with 5-aminolevulinic acid (ALA) production. Photosynthetic bacteria was isolated from a variety of habitats, ALA content was detected by spectrophotometer, the strain was identification by morphological analysis and 16SrRNA analysis, the gene hemA was detection by PCR. A strain RB with 5-aminolevulinic acid (ALA) production was obtained. It was Rhodopseudomonas palustris of Rhodopseudomonas with 4.34 mg/L ALA production. Moreover, the hemA gene fragment was amplified from the strain. The results showed that the photosynthetic bacteria with 5-aminolevulinic acid (ALA) production were obtained successfully. And also successfully obtained the hemA amplification products, which provide the foundation for constructing gene engineering bacteria.