Abstract:

Cytochrome C oxidase subunit I (COI) gene was amplified in 4 species of crane(22 individuals) by PCR with bird universal primers in order to study DNA barcodes of 9 species of crane.13 COI gene sequences of crane were downloaded from GenBank and BOLD Systems, totally 35 COI gene sequences were studied and build Neighbor-joining tree and Maximum parsimony tree to explore whether the DNA barcodes could distinguish between these species. The result shows that among the red-crowned crane group all were in the same class besides D1 and B18;in the group of white crane ,the individual codes AY567881 was in the same class of black crane; the result of phylogenesis analysis about other cranes was equal to the morphological classification result. The most possibility of the result maybe they were the same species or hybridize in related species because red-crowned cranes were all obtained from Shenyang Forest Wild Animal Zoo; The bigger difference in species of white crane probably cause by geography position or other reasons.