Abstract:

To realize the role of iron on the gene expression of plant, the proteome at different iron levels in wheat roots were explored by two-dimensional electrophoresis (2-DE). The wheat seedling with 3 leaves which grown at Hoagland solution were separated into two groups, the first group was kept at normal Hoagland solution till the end of the experiments, and the second was grown at the Hoagland solution without iron. As soon as the deficient iron symptom of the wheat plants appeared, half of them were harvested and at the same time some plants of the first group as a comparable sample was also harvested. The other half of the plants of the second group were supplied with normal Hoagland solution for one week and then harvested, at the same time the remaining plants in the first group were also harvested. The protein of every sample was only abstracted from the roots. After the proteins separated by 2-DE, the protein spots were analyzed by PDQuest software. The results showed that, comparable to the plants supplied with normal iron level, the difference of 137 protein spots from the treated wheat roots appeared significant level (P <0.05), 76 protein spots of them were up-regulated by more than two times, and other 61 protein spots appeared down-regulated by more than two times. In the second experiment, 150 different protein spots appeared (significant level P <0.05) after the iron deficient plants were supplied with normal iron level for one week. 78 of them were up regulated by more than two times, and other 72 spots were down-regulated by more than 2 times.