Abstract:

Linamarase is a kind of enzyme, which can degrade Linamarin in cassava, croton and other crops. The structure of this enzyme is very similar with myrosinase. Our study on this enzyme was avail to announce the relationship between cyanogen glycosidase enzyme and myrosinase, at the same time also reveal the origin of myrosinase. Linamarase gene was cloned from cassava, the yeast expression vector was constructed and then transformed into pichia pastorios GS115. Purified recombinant protein of Linamarase was obtained after induction and affinity purification. SDS-PAGE analysis showed that, the recombinant protein concentrated was in the 71 kD molecular weight around. The optimal temperature of this enzyme was about 37℃, and the optimal pH was about 5. The Km was 1.70 mmol/L, and Vmax was 8.36 μmol/(min?mg), while pNPG was used as the substrate.