Abstract:

According to the situation of narrow genetic background in Chinese tobacco cultivars, the genetic diversity analysis of tobacco germplasm could provide important information for breeding and introducing cultivars, and germplasm resource protection. In this study, genetic diversity analysis for 50 tobacco cultivars collected from different countries/regions were performed by using fluorescence SSR technique with 20 SSR primer combinations, a total of 81 allelic variations among the collected tobacco germplasm were detected with an average of 4.1 alleles per locus of each SSR. The result showed that the SSR combination of PT20457 possessed the highest values of polymorphism information content (PIC) (0.944) and could be effective locus for identifying DNA variation among these tobacco cultivars. Based on the polymorphic data, fifty tobacco cultivars were classified into 3 groups by UPGMA method. Group I contains Yunluo-01 which was sun-cured tobacco and derived from Guangdong, group II contained Atnarello Maoliang and Brazil Shailiang which were burley, group III contains 47 tobacco cultivars which were derived from 4 different countries. By using principle coordinates analysis, these tobacco varieties were clustered into 4 groups and 8 subgroups. Both cluster analysis methods markers revealed the genetic diversity and genetic relationships among tobacco varieties, and could provide a scientific basis for genetic research and tobacco breeding.