Abstract:

For the method of rapid diagnosis of Ralstonia solanacearum contain in tobacco fields, this paper based on the comparison result of the five whole-genome of the published genomes of Ralstonia solanacearum, designed nine pairs of specific primers of Ralstonia solanacearum, and has achieved three pairs of primers for specific amplification of Pseudomonas solanacearum by screening and verification. The application of the screened primers could be used as an effective method to do specific detection of tobacco Ralstonia solanacearum in the soil. As a preliminary conclusion, the lowest detection rate of Ralstonia solanacearum in the soil was 2×106/g. Thus, it’s a rapid and accurate new way for detection of tobacco Ralstonia solanacearum in the soil.